Laboratories for chemistry and environmental technology

The Faculty of Science and Technology at UiS has several laboratories within the field of chemistry and environmental technology. Here is an overview of relevant equipment and facilities.

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Get to know our laboratories within chemistry and environmental technology.

NMR

Nuclear magnetic resonance spectroscopy (NMR) is an ideal analytical technique used for determining the content and purity of a sample and its molecular structure (mainly smaller organic molecules).

Laboratoriet blir brukt til både undervisning og forskning.

  • Ascend 400MHz NMR Spectrometer 
  • The 1D Proton Experiment.  
  •  Basic 13C Experiments.  
  •  13C Experiments to Assess Multiplicities: DEPT and APT.  
  •  Two-dimensional nuclear magnetic resonance spectroscopy. 

Contact person: Erling Berge Monsen

Respiromitry

Respirometry involves measuring and interpreting biological oxygen demand. Oxygen consumption is directly associated with microbial consumption of substrate. A respirometer is a device used to measure the respiration rate as a change in the amount of oxygen over time.

KjemiMiljø_respirimetri

Oxi-Top is used for static measurement of biological oxygen demand. Change in oxygen concentration is measured by pressure change in the system. 

Micro Oxy Max measures biological oxygen consumption dynamically. Oxygen change is analyzed by oxygen detector. 

BM-EVO2 is a laboratory analyzer of respirometry of two reactors that can run simultaneously and on independent form. BM-EVO2 can be a fundamental tool for design, modelling, control and protection of the biological process of a wastewater treatment plant (WWTP). It could also be used as screening instrument for industrial wastes and compost assessment. The respirometry principle is based on the oxygen uptake by the microorganisms contained in the genuine activated sludge from the own biological reactor of the treatment plant. 

 

  • Oxi-Top
  • BM-EVO2 Respirometer
  • Micro Oxy Max
  • Biological oxygen demand (BOD) 
  • Dynamic exogenous respiration rate. 
  • Specific Rs  
  • Consumed oxygen in the substrate oxidation 
  • Biodegradable fraction of COD 
  • COD utilization rate 
  • Specific COD utilization rate 
  • Spesifikk oksygenutnyttelsesgrad (OUR), tester med biomassebærere

Contact person: Ilke Pala-Ozkok

Mass spectrophotometry

Mass spectrophotometry is an analytical technique based on measurement of mass to charge ratio of the analyte. Quantitative and qualitative data may be obtained depending on instrumentation and methodology.

KjemiMiljø_massespektrometri

The technique is widely used is a detector as a part of a chromograph system but can also be used standalone. 

At UiS we have two GC-MS (single quadropole) systems and two LC-MS/MS (double) for separation and identification of samples. Our standalone electron spray MS (single quadropole) is used for quick identification of single components sample, either directly from a TLC plate or syringe injection. 

  • Advion Expression S MS with Plate Express® TLC Plate Reader 
  • Agilent 5973 as a part of a GC system 
  • Agilent 5975 as a part of a GC system 
  • Waters Quattro Premier XE MS/MS as a part of a Waters HPLC system 
  • Identification of substances, for instance drug detection, environmental analysis and identification of unknown samples.

Contact person: Kjetil Bårdsen

Chromatography

Chromatography is a technique used for separation of complex mixture into single components for identification or further analysis. Separation occurs because of the different components in a mixture interact with the mobile and stationary phase.

KemiMiljø_Kromatografi_MS

There are many types of chromatography, for example gas chromatography, liquid chromatography, size exclusion chromatography and ion exchange chromatography.  

  • Agilent 6890 GC-MS  
  • Agilent 6890 GC-FID  
  • Agilent 7890 GC-FID/TCD for gas analysis 
  • Agilent 1260 Infinity HPLC system with Wyatt MALS detector 
  • Thermo Dionex Ultimate 3000 HPLC with diode array detector 
  • Waters Acquity UPLC-MS/MS 
  • Waters 2795 HPLC with photodiode array detector 
  • ÄKTA start protein purification system 
  • Environmental pollutant analysis 
  • Biomolecules analysis 
  • Biogas analysis 
  • Polymer size determination 
  • Protein purification 

Contact person: Kjetil Bårdsen

Spectrophotometry 

Absorption spectroscopy is routinely used in analytical laboratories for quantitative and qualitative analyses. The technique is fairly simple, as light (UV, Vis or IR) is pased through a sample some of the light will be absorbed. The amount of light absorbed is detected.

spektrofotometer

At which wavelength and how much light is absorbed depends on the chromophores in the molecule. 

At UiS we can perform analysis with 1cm and 5 cm cuvettes, and on test tubes (~16mm). 

  • Full spectra analysis 
  • Single wavelength analysis 
  • Kinetic 

Contact person: Kjetil Bårdsen

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